Design and methods Antibodies

ثبت نشده
چکیده

Primary antibodies: Details of the antibodies used as primary reagents for immunostaining germinal center T cells are given in Table 1. The anti-SAP antibody used in this study was a commercial polyclonal reagent. A monoclonal antibody of the same specificity (anti-SH2D1A, Cell Signaling Technology, Inc, Boston, MA, Cat. No. 2805) was tested on tonsil sections and a limited number of diffuse large B cell lymphoma cases, and showed the same reactivity pattern as the polyclonal antibody.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Design and in Silico Construction of New Efficient Antibodies Against Gastric Tumor-associated Antigens Towards Introducing Combined Strategy for Vaccine Tumor Therapy

Introduction: Gastric cancer, as the second reason of cancer mortality around the world, is defined as one of the serious health problems and a wide range of current research is focused to overcome the challenges relevant to its successful therapy. Employing recent approaches in the field of vaccine tumor therapy and immunotherapic strategies such as utilization of monoclonal antibodies can pla...

متن کامل

Professional rational drug and biological product design methods based on chemoinformatics as a novel science

Background:                             The use of new technologies in the field of drug discovery and development plays an important role in introducing new and effective drugs, optimizing the properties of existing drugs and, in general the development of various drug-dependent industries, as well as the general health of human, animal and plant communities. These methods provide new drugs at...

متن کامل

Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene

Background: The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Objectives: Development of antibodies against extra cellular domain of influenza M2 (M2e) protein in egg yolk of laying hens. Methods: A Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e ...

متن کامل

Recombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii

Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Reco...

متن کامل

Main Quality Attributes of Monoclonal Antibodies and Effect of Cell Culture Components

The culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) approach defines the assured quality of the final product through the development stage. An important step in QbD is determination of the main quality attributes. During the media optimization, some of the main quality attribute...

متن کامل

Application of Specific Monoclonal Antibodies for Characterization of Leishmania spp. Promastigotes Using Indirect Immunofluorescent and Immunoperoxidase Tests

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).   Methods: Ap...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007